Summary: In this review we discuss how the improved TAG (triacylglycerol) synthesis model (including acyl editing and new enzymes such as PDCT) may be utilized to achieve the goal of effectively modifying plant oils for industrial uses. Based on the results of the last 20 years, creating a high mFA (modified fatty acids) accumulating plant will not be done by ...
Summary: Omega-3 long-chain polyunsaturated fatty acids are of considerable interest based on clear evidence of dietary health benefits and the concurrent decline of global sources (fish oils). Generating alternative transgenic plant sources of omega-3 LC-PUFAs has previously proved problematic. Here we describe a set of heterologous genes capable of efficiently directing synthesis of these fatty acids in the seed oil ...
Summary: New and sustainable sources of long-chain omega-3 oils containing DHA (docosahexaenoic acid, 22:6Ο3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG). The DHA content was 6.8% of total fatty ...
Summary: To improve oil quality and other agronomic characters, we have developed an efficient and simple in planta method to generate transgenic camelina plants. We also demonstrated that transgenic camelina seeds produced novel hydroxy fatty acids by transforming a castor fatty acid hydroxylase. Our results provide a rapid means to genetically improve agronomic characters of camelina, including fatty acid profiles ...
Summary: It is desirable to increase the content of the monounsaturated oleic acid and decrease the content of the polyunsaturated fatty acids, linoleic acid, and Ξ±-linolenic acids in camelina oils to improve oxidative stability. Desaturation of oleic acid to polyunsaturated acids is mainly controlled by the gene FAD2. Three FAD2 genes were identified in Camelina, with very high sequence similarity ...
Summary: Study investigates the distribution of triacylglycerols and their phospholipid precursors within cotyledons and the hypocotyl of generic C. sativa and genetically modified lines with altered seed lipid composition. The results reveal previously unknown differences in acyl lipid distribution in Camelina embryos and suggest that this spatial heterogeneity may or may not be able to be changed effectively in transgenic ...
