Summary: Protoplast electrofusion was used to create intertribal hybrids of Brassica napus and Camelina sativa. Seeds of hybrids had a modified fatty acid profile, indicating higher level of linolenic and eicosanoic acids than those of B. napus. Our results suggest that somatic hybridization offers opportunities for transferring entire genomes between B. napus and C. sativa in breeding for rapeseed improvement. ...
Summary: Transgenic Camelina plants overexpressing Arabidopsis MYB96 exhibited drought resistance by activating expression of Camelina wax biosynthetic genes and accumulating wax load. The results indicate that MYB96-mediated transcriptional regulation of wax biosynthetic genes is an approach applicable to generating drought resistant transgenic crops. Transgenic Camelina plants with enhanced drought tolerance could be cultivated on marginal land to produce renewable biofuels ...
Summary: Under controlled environmental conditions, overexpression of AtPAP2 in Camelina sativa resulted in longer hypocotyls, earlier flowering, faster growth rate, higher photosynthetic rate and stomatal conductance, increased seed yield and seed size in comparison with the wild-type line and null-lines. Similar to transgenic Arabidopsis, activity of sucrose phosphate synthase in leaves of transgenic Camelina was also significantly up-regulated. Changes in ...
Summary: In this review we discuss how the improved TAG (triacylglycerol) synthesis model (including acyl editing and new enzymes such as PDCT) may be utilized to achieve the goal of effectively modifying plant oils for industrial uses. Based on the results of the last 20 years, creating a high mFA (modified fatty acids) accumulating plant will not be done by ...
Summary: Omega-3 long-chain polyunsaturated fatty acids are of considerable interest based on clear evidence of dietary health benefits and the concurrent decline of global sources (fish oils). Generating alternative transgenic plant sources of omega-3 LC-PUFAs has previously proved problematic. Here we describe a set of heterologous genes capable of efficiently directing synthesis of these fatty acids in the seed oil ...
Summary: New and sustainable sources of long-chain omega-3 oils containing DHA (docosahexaenoic acid, 22:6Ο3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG). The DHA content was 6.8% of total fatty ...